APCI background vs esi?
Posted: Sun Jun 12, 2022 1:33 pm
Hi all,
Another newbie’s question…
Is the sensitivity in APCI (much) lower than in ESI?
I’ve been playing with a brand new DUIS source with our new HPLC MS and the background noise is quite high when I scan m/z 100 to 450.
When I inject a 1uL of a sample of amine (about 0.5mg/ml) on the C18 column (150x4.6 5um) I get close to saturating the detector, yet my ratio signal/noise is only about 7… is that normal when you have an APCI (via the DUIS) ?
I was thinking about it over the weekend and I wonder if it’s because the APCI is the noisy culprit…
I will check my solvents by flowing pure CH3CN and water directly out of the bottles to see if the issue is coming from there.
Another newbie’s question…
Is the sensitivity in APCI (much) lower than in ESI?
I’ve been playing with a brand new DUIS source with our new HPLC MS and the background noise is quite high when I scan m/z 100 to 450.
When I inject a 1uL of a sample of amine (about 0.5mg/ml) on the C18 column (150x4.6 5um) I get close to saturating the detector, yet my ratio signal/noise is only about 7… is that normal when you have an APCI (via the DUIS) ?
I was thinking about it over the weekend and I wonder if it’s because the APCI is the noisy culprit…
I will check my solvents by flowing pure CH3CN and water directly out of the bottles to see if the issue is coming from there.