separation of diammoniumcations
Posted: Mon Dec 13, 2004 7:27 pm
by schnippy
I try to find a suitable method for the separtation of diammoniumcations. These products were formed in an benzylation of diamines. I'm interested in a separation an analytical (HPLC) and also in a preparative scale. Has anybody an idea?
Posted: Tue Dec 14, 2004 12:10 am
by Uwe Neue
A reasonable standard application for a similar problem is the analysis of paraquat and diquat. Several methods are around, including MS-compatible methods. The simplest one uses an ion-pair reagent. Since you want to do prep, you probably want to work with a high ion-pair reagent concentration to obtain a good loadability. The disadvantage of this method is that you get your amine with the ion-pair reagent as the counterion.
An alternative method for the paraquat-diquat separation uses silica as an ion-exchanger, i.e. with a reversed-phase solvent.
Both methods have been developed at Waters, and you should be able to find at least the first method in the Chemistry eApplications Notebook.
Posted: Tue Dec 14, 2004 4:41 am
by SIELC_Tech
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