Chromatography Forum

Hello!

I am beginner, only start to understand all processes and spectrometry subtleties. Therefore in something I can be mistaken!

Before me there is now a problem definition of impurity in pesticidal preparations. It can be solvents, dyes etc. Research is spent on TOF-MS detector (Q-star Elite), ESI. A mobile phase 0,1%HCOOH/CH3CN=50/50
Flow = 0,2 ml/min
Tell me please, in what way I can define or at least see 1-Butanol? I try to carry out the analysis with a column and without. Do not see I peak m/z=75 (((

Can be it is necessary to use a column with a gradient? What column preferable to such analyses?

Whether there is for someone an experience of such researches? (Research of impurity)
Thank you very much!

Butanol will be much more easily done by GC than by LC.

Peter

Thank you for your reply!
I understand that GC is more convenient! But the work is only used LC / TOF-MS.

The first thing to do would be to set up an infusion experiment where you pump a dilute solution of butanol in your mobile phase directly into the interface. Either monitor at m/z=75 or scan around that value and tune your ESI parameters until you get a signal. That will establish that the MS part of your system is working. Only then should you worry about the LC part (isocratic or gradient).

Ок, Tom Jupille.
Thank you very much!
I will try tomorrow to get a signal by changing the settings ESI. Understand that the first i must just get a signal to the MS, and then had to think about LC parametres.