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Disodium Pamidronate method for cleaning validation
Posted: Mon Dec 13, 2004 9:12 am
by JM
Dear all,
I am looking for a sensitive method for pamidronate analysis up to 0.2 ppm. Since most of the methods are on RI not of mush use. could you suggest any better method??
Posted: Mon Dec 13, 2004 6:36 pm
by Steve
JM
We did some work on pamidronate, but not at that ppm level with fluorescence detection.
Here is the web page:
https://www.macherey-nagel.ch/web/MN-WE ... =HPLC00852
Posted: Tue Dec 14, 2004 9:12 am
by JM
Thanks steve,
But i need detail conditions on derivetization, can you pls help me??
JM
Posted: Tue Dec 14, 2004 1:39 pm
by Steve
Sorry I don't have any of that information.
OPA post-column reaction
Posted: Tue Dec 14, 2004 11:20 pm
by Einar Ponten
This highly hydrophilic amine should easily be retained on a ZIC®-HILIC column at high ratio of acetonitrile. Then a post-column reaction between OPA (o-phthalaldehyde [643-79-8]) and the amine enable sensitive flurescence detection.
I don't have the method, but it should be quite straightforward.