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Regarding protein hydrolysis

http://www.chromforum.org/viewtopic.php?t=115114

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Regarding protein hydrolysis

Posted: Sat Dec 11, 2021 10:03 am
by rajanpatel
Hello,

I have food sample which is in form of powder. I want to analyse the amino acid profile in this food sample. For protein hydrolysis via 6N HCL,

1. how can I create oxygen free environment in the reaction tube?
2. can I use reflux system for hydrolysis because it involves 110℃ for 20-24h?

Re: Regarding protein hydrolysis

Posted: Mon Feb 07, 2022 6:38 pm
by Jake
1. how can I create oxygen free environment in the reaction tube? Use a Firestone valve, or similar device

2. can I use reflux system for hydrolysis because it involves 110℃ for 20-24h? No. Depending on the protein, you'll experience excessive degradation. Protein hydrolysis is usually done in a sealed tube.

Re: Regarding protein hydrolysis

Posted: Wed Oct 09, 2024 9:19 am
by rajanpatel
which type of sealed tube do you have used?

Re: Regarding protein hydrolysis

Posted: Tue Oct 15, 2024 9:19 pm
by Jake
Just search "vacuum hydrolysis tube," and search for the size, design, and availability in your location.

Something like this:

https://www.dwk.com/na/kimble-kimax-vac ... lysis-tube
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