Chromatography Forum

After running a sequence in Chromeleon, the software is separating my later standards (all listed as Calibration Standards) into separate curves.

Ex: Standards 1-4 are being calibrated together, however 5 & 6 are each appearing by themselves.

I have checked that the same processing and instrument methods are applied to all of these injections, and cannot find any other differences that would cause this.

Has anyone seen anything similar before, or know of a way to correct this so the standards are all included in the same curve?

Did you assigned the levels of the calibration standards correctly?

Also it is bit confusing of what is the problem actually. Do your standard runs are seperated or the standard components?

In addition to marking each calibration type you also need to mark the level in the sequence. I suspect you are missing level 5 and 6 in the sequence. ( drop downs)
Also the component table (yellow boxes) must have concentrations populated. If this table is missing cal 5 and 6, then you know the levels are not properly identified in sequence and its an easy fix