Ideal column selection.
Posted: Mon Dec 06, 2021 3:01 pm
by Sure1234
I was hoping for some advice. I am currently looking for a new column for 2 new projects. One is for the separation/quantification of all the B vitamins and (annoyingly) sodium ascorbate. The second if for short chain fatty acids and other similar compounds, I would need decent separations of structural isomers (e.g. butyric and iso-butyric acid).
Do people think there is a column that will successfully work for both?
Re: Ideal column selection.
Posted: Tue Dec 07, 2021 5:33 pm
by Vlad Orlovsky
your best choice for analysis is mixed-mode columns. Here are few applications:
https://helixchrom.com/compounds/niacin ... dal-column
There are no short fatty acids, but if you use pH around 4-5, they will be ionized and retained. The most important question is what is your intended detection technique? I don't know if you need to quantify sodium, but UV (alone)
might not work well.
Re: Ideal column selection.
Posted: Wed Dec 15, 2021 2:32 pm
by MSCHemist
Short chain fatty acids would be better to use GC-FID or MSD. They make Free fatty acid columns or you could do a headspace esterfication like I do.
If you insist on doing it on the HPLC I assume anion exchange with a conductivity detector would probably work best?