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Perkin Elmer LC-55

Posted: Thu Oct 08, 2009 7:18 am
by herbmaker
I have a small self-financed home lab with lots of very old surplus instruments. I am also able to make basic refurbish work and also construct some new equipment. I have Perkin Elmer LC-55 spectrophotometer which I planned to use as VIS-detector in my HPLC system (now at 450nm); I have a separate UV detector. Now the baseline of the spectrophotometer is rising up all the time during the measurement even if there is no solvent flow on in the system. I think the problem is in the electronics. Could anyone give me suggestions which circuit component could cause the trouble? I know my question is quite specific but I ask anyway hoping that some old repair man could read this and give some suggestion based on practical experience.

Posted: Thu Oct 08, 2009 2:22 pm
by HW Mueller
What is in the cell during registering this rise?

Posted: Tue Oct 13, 2009 3:05 am
by herbmaker
In the cell there is solvent. In this application 10%acetone in methanol. There is no difference whether the solvent is flowing or not.