Chromatography Forum

Good evening,
Can anyone help me out here with some preliminary information about what's the difference between N-terminal protein sequencing and peptide mapping? are they synonoms of the same analytical technique? Also can someone recommend any useful links/forums or ebooks that offer a simplified introduction to these topics? I'm specialized in protein (as a whole unit if that's correct to say) quantification by HPLC (RPC, SEC)but not really oriented about analysis its single peptides and amino acids.... your input will be highly appreciated

N-terminal sequencing refers mainly to the (low-throughput) Edman sequencing while peptide mapping refers usually to the (high-throughput) mass spectrometry techniques for protein/peptide identification. A google or wikipedia search of these new terms will give you all the information that you want. If you still want more info on the techniques I will post some once I come back from travel...

Thank you very much for your reply. Actually I already tried to google these topics and came out with a bunch of specific articles explaining the usage of these techniques for analysis of specific substances. The only helpful "general" explanation was a page on Wikipedia for protein sequencing but nothing for peptide mapping.

The point is I'm trying to prepare a simplified presentation for my colleages at work to just introduce them to these topics (being an HPLC specialist), but we don't apply these types of analysis in our lab..

If you help me out with an introductary article/link or ebook to help me understand these techniques and their varities step by step I would be very thankkful.

Thanks in advance