ESI of protected Arginine peptide
Posted: Mon Sep 14, 2009 8:23 pm
dear All
I have a peptide of which the only residues are Arginie (7 total ) and Glycine (2 total). The side chains of the Arginine (guanidine) was protected by pbf.
I need to do some modifications to this peptide and plan to use ESI to monitor the reaction progress. As the peptide is not dissolved in water. I am using DMF (dimethylforamide) as solvent.
When I tried to measure ESI, I diluted the peptide/DMF solution 10 times using water, and injected into ESI. However I could not see any expected peaks.
My question is: can I use ESI to detect this peptide ? As the side chains of the Arginine was protected by pbf, is the peptide ionizable under ESI condition? Does DMF do anything bad to the ESI?
I have a peptide of which the only residues are Arginie (7 total ) and Glycine (2 total). The side chains of the Arginine (guanidine) was protected by pbf.
I need to do some modifications to this peptide and plan to use ESI to monitor the reaction progress. As the peptide is not dissolved in water. I am using DMF (dimethylforamide) as solvent.
When I tried to measure ESI, I diluted the peptide/DMF solution 10 times using water, and injected into ESI. However I could not see any expected peaks.
My question is: can I use ESI to detect this peptide ? As the side chains of the Arginine was protected by pbf, is the peptide ionizable under ESI condition? Does DMF do anything bad to the ESI?
