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- Posts: 2
- Joined: Mon Sep 14, 2009 12:35 am
Greetings,
I have an old Varian modular system - computer (windows 98) running Star 5.5, a couple of 215 pumps, UV module (320) and am trying to add a new (used) 410 autosampler.
I've been trying to work through the Star manual, the autosampler manual, and the 215 manual, but I'm a bit stuck.
My autosampler is connected via flat (DB9?) cable in the same chain as the pumps, UV module, and computer. It also is connected via P1 outputs to the pump, doing a contact closure upon autosampler injection. (My manual autosamplers use the same method.) And it is connected via P2 to the aux (?) connection on the pump, which are supposed to signal it to start the next run, I think. (One of the three - another one is already used to communicate with the UV-vis.)
So here's my problem. As long as I click "run" on the computer to get the computer to "waiting for inject" (I have an "inject wait" on my first step of my method), then start in the autosampler window (on the computer), the first run starts fine. The autosampler does its thing, and starts the clock on the computer upon injection.
However, sample number 2 never starts. I've got a contact closure at the end of my current method, which I thought was supposed to trigger the autosampler to start, but that doesn't seem to be working. I've also tried touching the lines together manually (which would be the same as a contact closure), but neither seems to trigger the start of another run.
So, I could use some suggestions from folks using similar systems. What needs to happen at the end of my method to cause the second sample to get injected?
Thanks!
p.s. Yes, I have a call in to tech support @ Varian. It is not yet clear if they're going to provide support or not, since I bought the autosampler used (all I could afford), so I hope someone here can share some insight!
I have an old Varian modular system - computer (windows 98) running Star 5.5, a couple of 215 pumps, UV module (320) and am trying to add a new (used) 410 autosampler.
I've been trying to work through the Star manual, the autosampler manual, and the 215 manual, but I'm a bit stuck.
My autosampler is connected via flat (DB9?) cable in the same chain as the pumps, UV module, and computer. It also is connected via P1 outputs to the pump, doing a contact closure upon autosampler injection. (My manual autosamplers use the same method.) And it is connected via P2 to the aux (?) connection on the pump, which are supposed to signal it to start the next run, I think. (One of the three - another one is already used to communicate with the UV-vis.)
So here's my problem. As long as I click "run" on the computer to get the computer to "waiting for inject" (I have an "inject wait" on my first step of my method), then start in the autosampler window (on the computer), the first run starts fine. The autosampler does its thing, and starts the clock on the computer upon injection.
However, sample number 2 never starts. I've got a contact closure at the end of my current method, which I thought was supposed to trigger the autosampler to start, but that doesn't seem to be working. I've also tried touching the lines together manually (which would be the same as a contact closure), but neither seems to trigger the start of another run.
So, I could use some suggestions from folks using similar systems. What needs to happen at the end of my method to cause the second sample to get injected?
Thanks!
p.s. Yes, I have a call in to tech support @ Varian. It is not yet clear if they're going to provide support or not, since I bought the autosampler used (all I could afford), so I hope someone here can share some insight!
