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Temparature effect
Posted: Fri Dec 03, 2004 3:29 pm
by mohan
i would like to know effect of tempature on liuid chromatography,
please give me suggestions, i will apprisiate
Posted: Sat Dec 04, 2004 2:51 am
by Black Sunshine
In my application (normal phase prep.), temperature has a pretty large impact on my chromatography. The warmer the mobile phase, the faster the run. At the bare minimum, a controlled temperature will give you consistency in run times. Depending on the criticality of the project, I control the mobile phase temp and maybe the column temp.
Posted: Sat Dec 04, 2004 2:55 am
by Kostas Petritis
You need to control both the temperature of mobile and stationary phase otherwise you affect negatively your chromatography due to temperature gradients...at least in the analytical and capillary column scale...it is true that thinks are different for preparative chromatography...
Posted: Sat Dec 04, 2004 8:45 pm
by Black Sunshine
Temperature gradients have a large impact on peak shape as well.
Posted: Thu Dec 09, 2004 4:45 pm
by Gurunath
yes, very much. if you are thermosensile samples.also you need to mentain temp. of mobile as the viscoty of solvent plays important role in separation. for sample application like in clinical trials of plasma and proteins high temp. can degrade the sample matrix even though now days the laboratories are well equiped with air conditioners.
regards.
guru