Chromatography Forum

Hi all

This is my first posting to this forum, so I'm not sure that anyone will even be able to help me!!

Basically I have just moved jobs to where they use HPLC linked to LCSolution software, made by Shimadzu. I was using Chromeleon before, and I've been tasked with sorting the new software out to automate it a bit more. However, the manuals are rubbish!

Basically what I want to do is create a qnt file which will allow me to calculate concentrations by external standard automatically - but I can't see any options for entering weights or standard purities, let alone specify a calculation. Is my program just not set up correctly, or is this not a feature of the program??

Any help would be gratefully appreciated!!

"However, the manuals are rubbish! " - I always knew they weren't the best but I always thought they were usable

"Is my program just not set up correctly, or is this not a feature of the program??" - surely one can calculate these kind of things, I would use search option in help if I were you

You chose it in the method file in quantification or sth like this, I know you can do this but I have worked on that software some time ago so can't get clear instructions like click here then there

It is feasible for sure

Hi

How did you do that in Chromeleon? if there is just on standard substance, one could use the seqence columns 'Weight' and 'Dilution' as well as 'amount' and 'Resp. Factor' in the MTH.
We have usually two different substances per solution and I didn't find a suitable way without use of UDCs and Calculations in the report.

Alex

Open up the Post Run analysis module, load a data file, choose quantitative parameters from the menu, and fill in the blanks.

If you are in the US call the Shimadzu toll free number, follow the prompts to access customer service, and open a technical support call for a software question.

Oh dear, good luck. I found LCSolution really inflexible. To move from LCSolution to Chromeleon was a dream I would hate the thought of ever having to return.

Sorry that was not much help!

Dear know275:
I have not used LCSolution; however, I have used ClassVP 6.8 (Shimadzu). From the sale rep, V=Validation, P=Productivity.

ClassVP has many limitations. (My humble opinion, as an average user). I found that the ClassVP can generate reports of peaks, but can not calculate/report other info, e.g. concentrations . The reports (canned templates, can be modified) do not offer that capability, and they are dificult to modify. Shimadzu provided printed books (manuals) for hardware (supposedly run with a floppy, and saved to another FD). The manuals were not well translated from Japanese. There is one Help file for ClassVP, but it is not helpful for beginners. (This will be the last time I voice my frustration with ClassVP). I have also used Chromeleon for a few years now, and people in our lab love it better.

For a vivid illustration, ClassVP is like a Ford-T. It is functional, and can move you from point A to point B.
Chromeleon is like a Cadillac (I don't drive this luxury car); it has GPS, OnStar, heat/air, radio/CD, air bags/seat belts. It will give you a better experience.

Here is another comparison: the sophistication of ClassVP is like that of highschooler starting beginning "non-abstract" algebra. The sophistication of CM is higher, like someone vested with "abstract" algebra in groups/rings/fields & isomorphisms.

And again, this is my opin.