Eclipse XDB Vs Eclipse Plus

[Kwet]

Does anyone knows what's the difference between the Zorbax Eclipse XDB C18 column and the Zorbax Eclipse Plus C18 one?

It's not really explicit in the manufacturer information.

Thanks in advance for your comments.

[Mayank72]

Does anyone knows what's the difference between the Zorbax Eclipse XDB C18 column and the Zorbax Eclipse Plus C18 one?

It's not really explicit in the manufacturer information.

Thanks in advance for your comments.

Hello There !

Eclipse Plus C-18 ...........new generation column which has improved sep.capability !! changes in the reagents (trade secret ) for getting better endcapping of the free residual silanols apparently gives this column a better separation capability than its predecessors like the XDB ( stands for Extra Dense Bonding) If you wish to look at the published info you will notice the following

-Eclipse Plus C-18 has a pore size of 95 A , Eclipse XDB has 80 A
-Eclipse Plus C-18 has a % C load of of 08%, Eclipse XDB has 10%
-Eclipse Plus C-18 has a surface area of 160m2/g , Eclipse XDB has 180m2/g

.....Cheers !!

[SiliCycle]

I know better equivalent for Zorbax Eclipse and the Plus version. Those columns have not very good pore size, surface area and carbon loading. The SiliaChrom XDB, XDB1 and XDB2 are better replacement for HPLC chromatography. If you need more info, let me know

[Mayank72]

I know better equivalent for Zorbax Eclipse and the Plus version. Those columns have not very good pore size, surface area and carbon loading. The SiliaChrom XDB, XDB1 and XDB2 are better replacement for HPLC chromatography. If you need more info, let me know

Please do so ! My email i.d. is mayank72@gmail.com

Mayank Mathur

you can also connect with me on my linkedin profile

http://www.linkedin.com/in/mayankmathur2008

[mohan_2008]

Hi Charles,

What makes you say that the Siliachrom columns are better to Agilents -

They have SB, XDB and you speak of the same columns as available at Siliachrom.

It appears all the packing material is Silica just like Agilents - nothing special here. They have made a pretty good combination of pore size, surface area and carbon loading. Why is this not good?

I have been working with Agilents columns for past 10 years and no problems encountered. Infact, i am very happy with their columns.

If you want to sell your columns - you may have to justify the features that make them superior to Agilents - Not just saying they're no good!

We need to remember that this is a scientific forum and not a bargain market.

Regards,

[SiliCycle]

Hello,
Yes it's fair to explain why SiliaChrom is better than Zorbax Series. First of all, I worked many years for Waters Corp. and I using HPLC column for more than 30 years. My first good HPLC column in my life was Zorbax SB C18, and it was very good. Now with specific application such like low and high pH, low bleeding for LC-MS, highly aqueous mobile phase etc...End-users for HPLC need better column in order to do all new challenging applications. So the problem with Zorbax is the pore size, too low and also the surface area as well. In order to improve it, we manufacture here at SiliCycle the same chemistry than Zorbax but we improve pore size up to 150A and surface area to at least 400m2/g (as Phenomenex does for surface area). Zorbax is on the market for a while but this product is still the same, never improved excepted for UHPLC recently for high pressure HPLC. But for regular Zorbax, the product is a very older product. In our lab, we tested SiliaChrom XDB2 compared to Zorbax SB C18, and the tailing factor for amitrytiline was 1.1 for SiliaChrom and 1.5 for Zorbax, also the plate count 9300 and only 7900 for Zorbax again. The SiliaChrom SB C18 has tailing factor as 1.0 for amitriptyline. The other thing, and based on my experience, HPLC columns is so expensive. I know that because I was end-user for many year and now I manufacture HPLC column. So the SiliaChrom Series is really not expensive compared to Agilent and Waters.
If you need more info, please let me know.
Kind Regards
Charles
Note: we have an application notes for LC-GC (September 2009), to demonstrate the high stability of SiliaChrom SB C18 at pH=1.0., good for acidic analytes and LC-MS users.

[HW Mueller]

It might be worthwhile to see

viewtopic.php?t=10417&postdays=0&postor ... e&start=15

and other similar chains.

[grzesiek]

let's not argue, let's see results, please post chromatograms and data so we all can learn and use new information for our benefit

[SiliCycle]

Thanks guys, I know is not easy for you to understand. Look application note in LC-GC September 2009.

[Uwe Neue]

So your packing has a specific pore volume of 2 mL/g?

[SiliCycle]

I cannot saw because patent pending for now. Sorry.

[Uwe Neue]

If this were the problem, than you should not sell it either, because it can easily be measured...

[SiliCycle]

No problem to measure because we have an international R&D team for silica gel. Lot of Ph.D only for material as silica with a strong physical chemistry background.

[mohan_2008]

Hi Charles,

Thanks for the explanation. But I am not really convinced.

1. How does increasing pore size from 80 to 150A improve separation chemistry?

2. How does increasing surface area improve your columns over Zorbax chemistry?

3. Your comparison test of a SiliaChrom XDB2 to a Zorbax SB C18 for tailing factor using a very strong base such as Amitryptiline does not add any significance to the performance of your column.
i) Reason, being you are comparing apples to grapes or something like that.
ii) Zorbax SB C18 columns are non-endcapped columns. They were deliberately made this way to withstand low pH applications (pH =2) since acids can chew off the end-capping. At the same time, since they are non-encapped, a strong base such as amitryptiline can have significant mixed-mode interactions with the residual silanols, which eventually lead to peak tailing (worse with strong bases).
iii) Your Silia XDB2 columns are "Xtra Densely Bonded" and probably double or triple endcapped which can virtually deactivate the stationary phase to any base interactions. In other words, due to a significantly lower number of residual silanols, amitryptiline can yield lesser tailing peaks.
iv) if you made this comparison study at neutral or basic pH the Zorbax SBC18 will tail even worser with amitryptiline, since at neutral pH the silanols tend to be very acidic.
v) Zorbax columns are not usable at pH less than 2, and hence comparison with Silia SBC18 columns at pH 1.0 does not seem to be fair, since at pH 1.0 you have suppressed all silanol influence and can get a better tailing factor.

Hence, I need to see a really good application, for eg, Silia XDB2 compared to Eclipse XDB and perform better. What is the end product chemistry of your Silia columns that may add a unique selectivity and robustness to the product.

Specially I need to know what is the chemistry that makes your Siliachrom usable even at pH =1.0. This might be an edge over zorbax since their low pH capability starts at pH =2.0.

[SiliCycle]

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