Chromatography Forum

hey all, I have a 5890 GC with chemstation. I am new to chemstation and we have revA0.9.01. Ive called agilent and they say that our version is too old to have a downloadable copy. So basically I am trying to create a calibration table and standard curve from 5 different standards using the tutorial alone. But Im still pretty new to running samples and calibrating. Im still not quite clear on how to quantify our sample or what I should do first.

Ive created a calibration sequence that does a heptane blank, the 5 standards, a reagent blank, and then another heptane blank. All the chromatograms look great, sharp peaks, little noise, no peaks where there shouldn't be, theyre all increasing in the amounts they should be . And I have figured out how to integrate the peaks and clean out the noise and auto scale. But I just need help for what comes next, any tips? Any links to literature that might be helpful. I did find one agilent e seminar that was pretty helpful. Thanks!

Hi,
Go to the calibration Menu item in data analysis,
select Calibration settings, in that window from the dropdown box, select "From data file",
fill in the rest of the information.
Load the Signal(s) for the Low Level Standard and Integrate
in the calibration menu, select new clibration table, then click the radio button with automatic setp, and have the number "1" in the level box, click ok
Fill in the Dialog, Fill in:Compound Name, Group, Amount
If the peak is the reference std then select "yes" under ISTD , and 1 under the #

now to load the second lowest standard, and click on Calibration, add level
and select level 2, etc...
when finished, go to calibration, calibration table options, to view and edit the table.
If you want some literature, you can email me , change the NOSPAM to chemstation => NOSPAM@gmail.com

Alex

hi thanks for the reply that basically answers my question, but for some reason in the calibration tab, the "add level" function is grayed out so i can't select it. For example i will load the first signal for standards level 1, make the table, save the method, then open up the second signal and the option is grayed out.