Cyano column in normal phase HPLC

[pipettemonkey]

A brief search in the literature turned up one study where cyano columns were tried in normal-phase mode to separate peptides. The peptides did not bind the column. This article was published 10 years ago.

J Chromatogr A. 1999 Apr 23;840(1):1-9.

Does anyone have experience separating peptides/proteins using cyano support in normal phase LC?

[Uwe Neue]

Some people call HILIC "aqueous normal phase" chromatography. If you mean this, please find the addres to a presentation that contains a peptide example in HILIC.

http://www.waters.com/webassets/cms/lib ... a20753.pdf

[pipettemonkey]

I was looking for an alternative to reverse-phase as another level of separation with different selectivity. The target peptides elute with 30-40% MeCN on C4 columns during reverse-phase mode. I'm not sure that would be considered a very "polar" peptide.