As far as I can tell by the picture you have removed the autosampler-head in order to change te syringe. Unless something is wrong this is not necessary.
The standard procedure would be:
- have the autosampler park its tool:
* I use Exalibur and when in the sequence-vieuw you have a pane on the left where your instruments are.
* click on the autosampler. -> you should see the status of the autosampler and have a button 'terminal'. click on it. you will see a virtual controler for the autosampler. If you have this controler on your autosampler (optional) you can use this one as well it is identical
*scroll down the menu to select 'RobotArmLeft' by clicking it.
* click options and select "park tool".
* select "RobotArmLeft" and then "options" again and select "move to home".
you can now acces the tool that has been parked and proceed with removing the syringe.
- take the tool from the toolstation, unscrew the black retaining nut that is holding the syringe and then you can remove the syringe.
- simply replace the syringe, put it back in the tool and then put the tool back into the toolchanger.
- when starting your analysis the autosampler will automatically pick up the correct tool.
maybe this can help:
https://assets.thermofisher.com/TFS-Ass ... 640-EN.pdf
pag 53 and probably best in your case is pag 151
