Doxorubicin Epirubicin Daunorubicin
Posted: Mon Aug 24, 2009 5:55 am
by Merrin
Hi everyone,
I desperately need some help with an assay I am attempting to develop. I am trying to separate the above compounds using a Cyclobond I 2000 column and fluorescence detection (ex 480, em 560). The MP is 30% ACN, 70% 50mM Na2PO4, pH 5.6. Flow rate 1.0 mL/min. No matter what I do e.g. changing pH, MP composition etc will sepaarate them - they all result in a single peak. Any suggestions??
Thanks.
Merrin
Posted: Mon Aug 24, 2009 9:27 am
by virtu
This SP with beta-cyclodextrin (test this: 50mMKH2PO4 5%v MeCN or 10mM ammonium acetate 75-85% MeCN, but you must chek stability of SP in last MP).
Change SP => Sil C18
Journal of Chromatography B: Biomedical Sciences and Applications
Volume 707, Issues 1-2, 10 April 1998, Pages 219-225
High-performance liquid chromatographic method with electrochemical detection has been developed for the simultaneous determination of epirubicin, 13-S-dihydroepirubicin, doxorubicin and 13-S-dihydrodoxorubicin in human plasma. An aliquot of 200 μl plasma, spiked with internal standard, was extracted by solid-phase extraction using polymeric adsorbent columns. Chromatography was performed using a C18 reversed-phase column with a mobile phase consisting of water–acetonitrile (71:29, v/v) containing 0.05 M Na2HPO4 and 0.05% v/v triethylamine adjusted to pH 4.6 with citric acid. Linearity of the method was obtained in the concentration range of 1–500 ng/ml for all the analytes. Analytical recoveries of the analytes ranged from 89 to 93%. The assay can be used for the simultaneous determination of the four analytes, or for epirubicin and its metabolite or doxorubicin and its metabolite, using the other parent drug as an internal standard. The method was applied to analyze human plasma samples from patients treated with epirubicin using doxorubicin as an internal standard.