UltiMate 3000 Flow Splitter
Posted: Thu Jun 24, 2021 7:31 pm
Hello everyone,
I'm trying to put an HPLC instrument built in 2005 into use and facing an issue regarding the flow splitter.
This instrument is a Dionex UltiMate 3000 this has 2 pumps: one regular flow and the other microflow.
To achieve nanoflow, they installed a flow splitter in the flow manager unit, that splits the flow 1:1000 to achieve nL/min flow rates.
The flow splitter has one input and two outputs. There is a main output and a second output. There is a loop between the two and there is another line that goes to the back of the instrument where they placed a flow control unit and a waste outlet.
When I start the flow, I don't see any solvents coming out of the line that is supposed to be the nanoflow line.
I was wondering if there is anyone familiar with these splitters?
Is it possible that there is a buildup in the splitter?
There is no solvent coming out of the "second" outlet of the splitter. The manual says there should be a very minor flow coming out of it, while a higher flow is coming out of the "first" outlet of the splitter.
I'm trying to put an HPLC instrument built in 2005 into use and facing an issue regarding the flow splitter.
This instrument is a Dionex UltiMate 3000 this has 2 pumps: one regular flow and the other microflow.
To achieve nanoflow, they installed a flow splitter in the flow manager unit, that splits the flow 1:1000 to achieve nL/min flow rates.
The flow splitter has one input and two outputs. There is a main output and a second output. There is a loop between the two and there is another line that goes to the back of the instrument where they placed a flow control unit and a waste outlet.
When I start the flow, I don't see any solvents coming out of the line that is supposed to be the nanoflow line.
I was wondering if there is anyone familiar with these splitters?
Is it possible that there is a buildup in the splitter?
There is no solvent coming out of the "second" outlet of the splitter. The manual says there should be a very minor flow coming out of it, while a higher flow is coming out of the "first" outlet of the splitter.