Chromatography Forum

Hi guys,

Have anyone tried before flow injection analysis of LCMSMS to optimise the fragmentor voltage?

Is it that instead of a column, we connect a nut in between and run standard solutions at different fragmentor voltage?

Thank you for the replies.

Regards,
hts123

Yes. In flow injection analysis, you place an union instead of the column (or a tubing that directly connects the HPLC injector to the MS) and make injections of a certain volume of standard solution (typically the injection volume that you are going to use in LC-MS) at regular intervals while modifying parameters. This provides a gain of time compared to LC-M injections. This is different from MS infusion where you would infuse a solution at a low flow rate in the MS using a syringe pump.

Usually, people first start with MS infusion to optimize compound-dependent parameters (e.g. collision energy, fragmentor), then move to flow injection analysis to optimize source parameters (desolvation temperature and flow, ESI voltage etc.).