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linearity problem

Posted: Wed Aug 12, 2009 5:50 pm
by subathrai
am trying to develop a uv and rp lc method in glimepride (2 mg)+rosiglitazone (2 mg)+metformin (500mg)bilayer tablet
my doubts -
1. suggest me a linearity range
2. in this metformin is sustained release and the rest is immediate release; this may produce any problem in the method?
3. what are the possible methods to develop methods in uv and rp lc : any suggestions?

Posted: Thu Aug 13, 2009 8:17 am
by grzesiek
1. ICH Q2(R1) should have this kind of info
2. should have not for HPLC
3. ? literature search, trial and error, scouting gradients, DOE ...