interaction between C18 stationary phase with HPMC and PVP?
Posted: Wed Apr 28, 2021 7:33 pm
Could this situation be an interaction between stationary phase and excipient?
I’m currently running 6 formulations that contain combinations of HPMC-AS and PVP-VA64 (or Kollidon VA64). I’m currently using an Agilent Infinitylab Poroshell 120 Bonus RP (P/N 695968-901) column (4.6 x 100mm; 2.7um particle size).
I noticed that the retention time of the NCE in my standards have shifted after I ran my 6 formulations. Can the combination of HPMC-AS, and or Kollidon VA64 being deposited on the head of my LC column be causing the retention time of my active to shift? Note, I am not using a pre-column.
I’m currently running 6 formulations that contain combinations of HPMC-AS and PVP-VA64 (or Kollidon VA64). I’m currently using an Agilent Infinitylab Poroshell 120 Bonus RP (P/N 695968-901) column (4.6 x 100mm; 2.7um particle size).
I noticed that the retention time of the NCE in my standards have shifted after I ran my 6 formulations. Can the combination of HPMC-AS, and or Kollidon VA64 being deposited on the head of my LC column be causing the retention time of my active to shift? Note, I am not using a pre-column.