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sugars by gc

Posted: Tue Aug 04, 2009 7:20 pm
by vestelshirley
I'm told there exists a derivitization method for sugars so that they can be analyzed by gc. Might anyone know of this procedure and, if so, the specifics method?

Posted: Tue Aug 04, 2009 8:09 pm
by chromatographer1
Silylating reagents work nicely for sugars.

I suggest you call Sigma Chemical or Pierce Chemical and ask their technical service departments for literature and a choice of reagents.

Sweeley reagent composed of dry pyridine, hexamethyldilazane, and trimethylchlorosilane is a generic reagent for silylating sugars.

Of course, the devil is in the details which are unknown. Time to have a nice chat with technical service.

best wishes,

Rodney George
consultant

Posted: Wed Aug 05, 2009 2:19 am
by Consumer Products Guy
We dissolve in DMF and derivatize with BSTFA.