Fatty acid synthesis - Detection of small compounds
Posted: Thu Jul 30, 2009 8:08 am
Hi everybody
I got a small problem trying to satisfy the wishes of my supervisor.
So far I did some analysis with the GCMS, mainly fatty acid composition and
quantification of a small reactive aldehyde. We would now like to investigate into earlier steps of the fatty acid synthesis.
Therefore I want to add to my organism radioactive labeled precoursers and then check where they are incooperated to. Now my boss just said:
So, the final question:
Any suggestions how to detect best small molecules like
I got a small problem trying to satisfy the wishes of my supervisor.
So far I did some analysis with the GCMS, mainly fatty acid composition and
quantification of a small reactive aldehyde. We would now like to investigate into earlier steps of the fatty acid synthesis.
Therefore I want to add to my organism radioactive labeled precoursers and then check where they are incooperated to. Now my boss just said:
After a lot of investigation I couldnt find anything suitable yetNo problem, just look for a GCMS protocol.
So, the final question:
Any suggestions how to detect best small molecules like
- - Pyruvate
- acetyl-coa
- malonyl-coa