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Posted: Tue Jul 28, 2009 8:07 pm
by shahis77
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Posted: Thu Jul 30, 2009 8:05 pm
by MaryCarson
Do you know whether your parent compounds even elutes with your mobile phase conditions? The parent is quite likely more hydrophobic than its oxo metabolite. If you need 90% MeOH to bring the metabolite off a C18 column, maybe you should try a C8?
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