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hplc method development

http://www.chromforum.org/viewtopic.php?t=10964

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hplc method development

Posted: Tue Jul 21, 2009 6:48 pm
by ajey
Please let me know that, what is the systematic procedure to develop hplc method?
Thanks & Regards,
Ajay

Posted: Wed Jul 22, 2009 2:27 am
by JGK
You are asking a great deal which is not something that can be easily crammed into a post on a message board.

I suggest you consult some reference material such as:

"Practical HPLC Method Development", 2nd Edition L. R. Snyder

also John Dolan's seven-part article "The perfect Method" on the LC GC North America site

as a starting point.

Posted: Wed Jul 22, 2009 2:55 am
by Vlad Orlovsky
Here are few guides we have for our mixed-mode columns. One will ask you bunch of questions and then show you application, column and conditions. Another one are couple of tables:

http://www.sielc.com/MethodDevelopment_ ... dType.html
http://www.sielc.com/MethodDevelopment_Guide.html
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