Dilution integrity

[kishore1984]

why we will do this test ? and wat the reason for take that much dilution only?

[gxch43]

Confirm the ability to dilute samples above Upper Limit of Quantitation (ULOQ),

[JGK]

Testing dilution integrity during validation ensures that a known "sample concentration" above the ULOQ can be successfully diluted, analyzed and produce an original concentration result of acceptable accuracy.

If an initial "unknown" sample analysis is above the ULOQ of the assay it will need to be diluted. Armed with the dilution integrity data, you can be assured that, if the dilution factor is within the range tested in the validation, the diluted "unknown" will produce a result of acceptable accuracy.