offline HPLC chromatogram viewer

[mashtikar]

Hello

I am working in an University lab. We just completed a project for some company. We have generated a lot of chromatograms while running the experiments and now the company we worked for wants to see the chromatograms, they wish to review our chromatograms as they believe this data will help them in some other project they are working on.

I can give them the chromatograms but it would be useless for them since they dont have a single HPLC system with them and wont be able to open them.

I was wondering if there are any good offline chromatogram viewers available.


Shimadzu's Series 10 HPLC system was used to generate these chromatograms. Shimadzu uses an 'EZStart' application; I recently talked to an Shimadzu engineer and he said that EZStart will not work if an HPLC is not hooked to the computer. Now I am not sure how correct that is, but my attempts of installing the EZStart on some other computer which was not connected with the HPLC have failed.

The company just needs a viewer which will allow them to review the different peaks at different wavelengths. I have googled a lot and have found nothing useful.

Does anybody have any solutions?

Let me know if you guys need any more details.

Thanks a lot!

[danko]

Mashtikar,

It sounds very weird that it shouldn’t be possible to install and use a CDS without having an actual HPLC system connected to the computer. Do you need to turn your system on in order to review data or is the system only necessary under the installation/configuration procedure? I would expect the latter to be the case (if the necessity is there at all). And if indeed that is the case, why don’t you hook your customer’s PC/laptop to your HPLC, install the CDS and let them play around with the data you generated for them.
Remember to ensure some kind of clearance of the approach with the CDS’ provider.
Alternatively, why don’t you print the data they need and let them be happy?

Best REgards

[Csaba]

Danko:
"It sounds very weird that it shouldn’t be possible to install and use a CDS without having an actual HPLC system "

That requirement is one of my top 20 requirements when I evaluate an lab application. It is surprisingly many applications that you can't do a evaluation of data without the hardware.

Regards
Csaba

[HW Mueller]

The good old paper, how simple it was.
Seriously, there usually (always?) are some methods to transfer the data to another form, like ASCII, to export the data to another software.

Also, even my extremely old software allows to "contimue" without any HPLC detector turned on.

[sam.pedraglio]

Simply type www.google.it and search for ezchrom offline data.
It's easy to find that you can buy an off-line license of ezchrom, the engine behind ezstart.

[mashtikar]

Sam, I have already found that Agilent will sell me EZChrome Elite. But its not just the offline package. Its an entire CDS. So why will I not have same problem with the copy I already have with me?

Danko its only the installation which requires me have the HPLC hooked up. and if we install this software on the company's computers then we would be violating the license.

How about any third party software that know how to read the chromatograms generated by the Shimadzu HPLC? Has anyone heard about it before?

Thanks

[DR]

How about exporting chromatograms in ANDI (AIA) format? There are several readers for that (ChromMerge is an old, but good one).

[mashtikar]

DR, do you have any idea how to convert files into ANDI format?

EZChrom has an export option but apparently its will only export it into ANDI when the actual chromatogram is running. This export option is in the Method's section so it converts the std chromatogram into ANDI format after the run is complete.

But I already have all the chromatograms I need.

Do you know of any other converter?

Dont you just hate it when you run into such issues created by the software developers! (read that as policy makers)

[sam.pedraglio]

The site I found was www.chromtech.com and they speak about an off-line data analysis license and not a complete CDS. You need to have an on-line system (Serial number required).

[mashtikar]

Sam, Thanks for the link. Basically I was looking for something like PeakSimple (CDS from chromtech) but unfortunately my chromatograms wont open with it.

PeakSimple needs chromatograms to be in CHR or in ASCI format. And I have yet to reach a successful attempt at converting my chromatograms to any of the above formats.

In the mean time any pointers are welcome.

Thanks!

[Koen Hollebekkers]

What version of the Class-VP do you have?

Conversion to AIA should be possible, this format is normally importable in most software packages.

Do you have the license plugged in the PC where the data is stored on?

[mashtikar]

Koen, Its a Class VP-7. To be specific its the version 7.4SP1 of the EZStart.

And yes the license key is a small USB stub plugged into the PC that stores all the chromatograms. Does it matter? I dont really know.

[mashtikar]

Can anybody confirm if EZChrome Elite will be able to read chromatograms generated on Shimadzu Series 10 HPLC which uses EZStart version 7.4SP1?

Thanks a lot!

[Koen Hollebekkers]

Hmm,

I don't understand why your not able to install the software.

I would say that principally it should be possible to read files that have been generated with shimadzu systems. Of course the files need to be generated by class vp or ezlite 7.4. You also need a license on the PC where you want to open the data files.

Control with EZlite of shimadzu systems is only possible with a driver.

What type of communication module do you use?