Chromatography Forum

hello,

I am currently using a Corona CAD detector as well as a UV detector. Although I am seeing a good peak shape in my UV, I am seeing nothing in my CAD. I have tried changing all the parameters on the CAD, and even the concentrations of my sample. Could it be that my sample (2-hydro-3-pyridazinone is just not volatile. I am using a Diamond Hydride column with 97:3 ACN: 10mM ammonium acetate (aq) as my mobile phase. This is being ran isocratically.

thanks

Some tentative issues:

Do you inject enough to be able to detect it by CAD? Can you inject 10 times more on column?
Can you inject something else along to make sure that the tandem connection to the CAD is well done (alternatively by-pass the UV detector).
I do not know the volatility of your compound (probably no-volatile), but if it is semi-volatile it might be an issue.


Is there a good reason to try to this by CAD instead just UV?

I am using CAD because it is integrated in my summer project. I am a mere summer intern and am just trying to gain experience with different detectors, instruments, etc. Thanks for your suggestion regarding increasing the injection volume. I will try it.