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Nucleotide separation columns available commercially?
Posted: Fri Jul 03, 2009 1:06 pm
by msrvnn
For my experiments i need to separate all the nucleotide and hyperphosphorylated nucleosides from a cell extract.. Basically it would be nice if i can find a column which can bind these molecules and then can be eluted out later.. Is there a commercial chromatographic column which solves this purpose??
Posted: Fri Jul 03, 2009 1:47 pm
by Uwe Neue
Look for the Oligonucleotide Separation Technology (OST) columns at the Waters website. You will find plenty of application notes, or you can get a brief overview in the column catalogue 720002784en.
Posted: Fri Jul 03, 2009 2:42 pm
by R13a204
Is it true that OST columns are just your normal BEH columns with a blue label ?
Posted: Sat Jul 11, 2009 4:35 pm
by PHOBIUS
Alternativelly, you can use a CE method. There is quite a lot of papers dealing with analysis of intracellular nucleotides by CE. I found this one handy :
http://www.ncbi.nlm.nih.gov/pubmed/1720 ... d_RVDocSum