lost prime

[mago]

Can anyone please let me know what's lost prime?

[tom jupille]

"Priming" a pump refers to filling the inlet lines and pump head with liquid so that it can pump efficiently (i.e., so that there is no air in the inlet lines, pump head, or check valves). "Lost prime" means that a significant amount of air is trapped in the system such that it can no longer pump correctly.

[mago]

Thanks Tom Jupille your reply.
I just want to learn more from you. can you please help me to understand about lost prime problem?
My run stoped at the midle and i noticed the mobile A did not have enought, but some one told me that it cause by the lost prime, is it correct?

[Kostas Petritis]

It's the other way round. If your mobile phase A bottle is empty or the tubing is not correctly emerged in the mobile phase, that will introduced air in to the system and it can lead to "lost prime". HPLC software can detect extensive pressure variations or you can add a minimum back pressure at which point the method/pump will shut down.

[shahis77]

AIR IN SYSTEM..........MEANS LOST PRIME
Just purge the system 2-3 times
if not solved then open the screw below the pump till u get liquid there...