Air bubble in the Ion chromatography column

[sobhkuma]

Dear All,

I am working with very sensitive Ion chromatography. By mistake, I forgot to click on the auto shut off mode and it ran out of eluent overnight. I already have experience in dealing with such situations.
Unfortunately, I was overwhelmed today and without removing the air bubble from the pump head, I started the IC system with a new eluent. Until I realized my mistake it was late and I think there is already a bubble introduced in the column. I removed the bubble from the pump head but there is a disturbance in the baseline.
Pressure, retention time, and background conductivity are working fine, but there is a lot of disturbance in the baseline. I am worried if it can get back the same performance. It is an expensive and new column.

Any assistance in this regard will be helpful!
Many thanks!

[vmu]

Pump the eluent through the column to remove the air. When the baseline becomes stable, continue working.

[Multidimensional]

Not likely that you have a "bubble" in your column. Gases are easily compressed in liquid. Just prime the system, flush the column and make sure you are degassing your mobile phase properly.