blank for analysis of an endogenous compund in plasma?
Posted: Thu Jun 11, 2009 1:35 pm
I'm currently working on my masters degree in analytical chemistry and I'm going to measure concentrations of an endogenous peptide in plasma samples by LCMS.
My big problem now is to make a calibration curve when there's no blank. I thought about using standard addition, but because this peptide exist in such small amounts I have to use a lot of plasma every time I run the analysis, so unfortunately the amount of samples I have is not enough to use standard addition on everything, as well as it would be very time consuming.
I've thought about using synthetic plasma for the calibration curve, but can these results be extrapolated to real plasma?
If anyone have any suggestions on what to do I would be pleased to hear it. I feel kindof stuck on this matter right now.
My big problem now is to make a calibration curve when there's no blank. I thought about using standard addition, but because this peptide exist in such small amounts I have to use a lot of plasma every time I run the analysis, so unfortunately the amount of samples I have is not enough to use standard addition on everything, as well as it would be very time consuming.
I've thought about using synthetic plasma for the calibration curve, but can these results be extrapolated to real plasma?
If anyone have any suggestions on what to do I would be pleased to hear it. I feel kindof stuck on this matter right now.