Chromatography Forum

Hello,

I intend buying a Hamilton PRP-X100 column for analysis (non-suppressed) of the common anions in water. I have a choice of three types;

(1) Hamilton PRP-X100 5µ 4.6 x 150 mm
(2) Hamilton PRP-X100 10µ 4.1 x 150 mm
(3) Hamilton PRP-X100 5µ 4.1 x 100 mm

First off, I have no experience of using polymeric based columns, only silica ones. I am thinking about option (1) but I wonder what kind of back pressure would I get? Would it be excessively high? Any thoughts of members who have experience working with these columns would be very appreciated

Thanks in advance
Pat Crowley.

We use Hamilton PRP-X100 here, mostly for sulfate assay. Our columns are a few years old, haven't purchased any recently. So, for us, I'd likely choose #3. We don't use any 10µ columns at all. The difference in length and i.d. between #1 and #3 will likely not be too significant. In general, we tend to be moving to smaller i.d. columns for all our HPLC assays, to save solvent costs, and even HPLC water has a cost.

Off subject - how did you make the "µ" symbol in this post? I copied and pasted yours into my response, and I'm not very good on computers.

Hello,

Thanks for the reply. On the subject of the micron symbol; I'm using a mac, and you get the µ symbol by typing alt-m. I don't know what the command for windows is, but google might track it down for your particular operatring system (if you use windows)

We would use the PRP-X100 column for undergraduate experiments, so I would like to have a column that would cover a wide arange of applications as possible (we are not tied to one particular assay). Solvent consumption is not a major concern for us, as the chromatograph would only be in use for a few weeks each semester.
I'm still wonder about the back pressure on the 5µ 150 mm column. Would it be similar to the back pressure on a regular 5µ c-18 silica culumn, using a high aqueous content?

Thanks again.

I checked our PRP-X100 columns, all of which are at least a decade old. One is 150mm x 4.1mm, and 10µ; another is 100mm x 4.1mm. Our pressures ran about 120 bar with aqueous KHP mobile phase, 1.00 ml/min, no pressure issues.

I would check with Hamilton and see the test chromatogram to see which of the columns suits best. I also would use 5 u columns (µ usually is <alt>0181).

As former member of the Hamilton Chromatography team I would like to add that the 4.6mm i.d. column uses PEEK hardware, the 4.1mm i.d. column is stainless steel (including the frits).

Send an e-mail to hplc@hamiltoncompany.com and ask. If you would like to talk to the guys in the lab contact smeikrantz[at]hamiltoncompany.com
They also have a revamped web site with improved search functions for application notes.
On Windows based computers the µ can be generated by holding the ALT key and typing 0181 (ALT0181). ASCII code comes in handy from time to time. You can do ®, ±, ², ³, Å, ß, °, ¼, ½, ä, ö, ü and so on...The character map tells you the ASCII codes.

cheers,

Robert

sorry Markus, I just saw that you already explained the ALT0181...