Prilates
Posted: Tue Jun 09, 2009 7:23 am
Hi,
We are trying to quantitate Pril/Prilates from human plasma.
Our problem is that the sensitivity for Prilat is much much lover than that of Pril. (Enalapril/Perindopril/Ramipril/Quinalapril).
We are using positive ESI with MS/MS detection, MeOH/H2O with 2.5 mM Ammonium Formate in mobile phase. We have varied the MeOH / H2O ratio
from 91/10 to 45/55, but no significant reponse enhancement, only better separation at lower Methanol ratio.
Also tried in negative ESI but no response.
If any of you has an idea for a better method please help.
Best Regards,
TR
We are trying to quantitate Pril/Prilates from human plasma.
Our problem is that the sensitivity for Prilat is much much lover than that of Pril. (Enalapril/Perindopril/Ramipril/Quinalapril).
We are using positive ESI with MS/MS detection, MeOH/H2O with 2.5 mM Ammonium Formate in mobile phase. We have varied the MeOH / H2O ratio
from 91/10 to 45/55, but no significant reponse enhancement, only better separation at lower Methanol ratio.
Also tried in negative ESI but no response.
If any of you has an idea for a better method please help.
Best Regards,
TR