Removing primuline from preparative TLC lipids ?
Posted: Mon Oct 12, 2020 2:40 pm
Hello,
I would like to make preparative TLC to separate my glycolipid sample. The aim is to obtaine different glycolipid subfamillies (i.e. MGDG, DGDG, SQDG...) from an SPE fraction which contained MGDG, DGDG, or eventually SQDG...
I used primuline to detect non-destructively the lipids. However, I wonder about the primuline's presence in the different scrabed bands.
Do any one know how to remove primuline without modifying the separated lipids ?
I thank you in advance for your precious help.
I would like to make preparative TLC to separate my glycolipid sample. The aim is to obtaine different glycolipid subfamillies (i.e. MGDG, DGDG, SQDG...) from an SPE fraction which contained MGDG, DGDG, or eventually SQDG...
I used primuline to detect non-destructively the lipids. However, I wonder about the primuline's presence in the different scrabed bands.
Do any one know how to remove primuline without modifying the separated lipids ?
I thank you in advance for your precious help.