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SEC MW standards/markers, ~5 kDa, preferably peptides
Posted: Tue Jun 02, 2009 6:07 pm
by Millahh
Does anyone know of some good, reasonably priced peptides in this mass range that I could use as a MW marker and in some recovery experiments in a validation? The methods is for determination of multimers of a 2.5 kDa peptide, so i'm looking for something ~5 kDa, and maybe something 7-8 kDa as well. No commercial SEC std kit i've found has anything as low as what I'm looking for, and looking up the MW of every known peptide, then pricing them seems a bit...inefficient.
Posted: Tue Jun 02, 2009 7:00 pm
by Vlad Orlovsky
Insulin can work for you. It is 5808....and you can get it even from drug store
Posted: Tue Jun 02, 2009 8:27 pm
by Millahh
Thanks!
Come to think of it, I need something around 3 kDa, as well (unfortunately, the B-chain of insulin is a bit pricey).
Posted: Tue Jun 02, 2009 9:07 pm
by Vlad Orlovsky
You can get insulin for Walgreens or other drug store for about $80 bottle. Bottle has 10 ml solution of insulin (about 3.5 mg/ml) plus other stuff (glycerol, polysorbate, etc.). You might have somebody with diabetes around who can donate you few ml.
Posted: Tue Jun 02, 2009 9:24 pm
by Millahh
Sigma had a good price...100 mg bovine insulin for $120, so i bought a few bottles.