quantifying analytes

[offroad]

Dear friends,

please, could you shed some light on this?

If on a chromatogram I have 3 peaks, A, B and C, and their area, x, y and z.

Would it be right and precise to say that the composition of the compounds is taken from the areas only?

Let x = 10, y = 20, z = 30

Composition of A = 10/(10+20+30) [percent] and so on?

Thank you

[Kostas Petritis]

Most likely not as the detector response for each of the analytes is probably different (and most times is). If the response of your analytes is the same or if you are using detectors with "equimass" or "equimolar" response you might be able to make "similar" claims.

[grzesiek]

preparing calibration curves using standards (again ) will let you quantify your components, it will also answer the question/doubt raised by kostas - whether response if different or not

marciobarbalho these are some basic questions you ask, and there is this student projects section for this

I still hope we helped

[offroad]

preparing calibration curves using standards (again ) will let you quantify your components, it will also answer the question/doubt raised by kostas - whether response if different or not

marciobarbalho these are some basic questions you ask, and there is this student projects section for this

I still hope we helped

Yes, basic question, I am preparing the standards, that was just a doubt, it seems the answer is 'no'.

[grzesiek]

yes, the simple answer is: "usually no"