Response Factor Masslynx

[Ekatz]

How would one verify that the Masslynx is calculating the Response factor, RRF SD and relative SD from a RF curve type? I know what MassLynx calculated, but how would I go about verifing it myself on a diffenent program?

[tom jupille]

First of all, you need to establish whether you're using a straight least-squares fit or a weighted least-squares fit. If it's regular least squares, you the RF is the slope of the fit, the RRF is the ratio of the RF of an analyte to (usually) the internal standard or another analyte. SD is the standard deviation, RSD is the relative standard deviation (SD divided by the mean value of replicate measurements). All of those can be generated easily in Excel.

[lmh]

and look into the linest() function in excel if you go that way.