Chromatography Forum

Posted: **Thu Apr 02, 2009 10:39 am**

Can we use Dichloromethane in supelcosil LC-18-DB column?

Posted: **Thu Apr 02, 2009 1:09 pm**

sure, why not? why would you?

Alex

Posted: **Fri Apr 03, 2009 3:51 am**

thanks alex,

one of our client wants to use this column with DCM and with a gradient program of,
Time (minutes) DCM, Methanol
0 10 90
20 10 90
55 50 50
60 50 50
60.1 10 90
70 10 90

Posted: **Fri Apr 03, 2009 9:41 am**

Be careful if you have PEEK parts in your hplc system. I believe PEEK may have compatibility problems with DCM, but I might be wrong.

Posted: **Fri Apr 03, 2009 12:21 pm**

I would ask the client to explain the method to me, and also I would ask him if he ever ran this method on more than one column.

Posted: **Thu Apr 09, 2009 6:25 pm**

Be careful if you have PEEK parts in your hplc system. I believe PEEK may have compatibility problems with DCM, but I might be wrong.

Imh has right. Mobile phases with % DCM higher than 5% can cause incompatibility issues with PEEK tubings or columns.

Posted: **Fri Apr 10, 2009 10:22 am**

If it is an Agilent system that you are using, the supply different piston seals for normal phase work.

Chromatography Forum

use of DCM

use of DCM