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- Posts: 26
- Joined: Tue Nov 26, 2019 10:39 am
I am doing reversed phase LC. I have had a sudden retention loss - by a couple of minutes.
We had an issue with the solvent running dry last week - i know, not the best. i rehydrated the column over a few hours at high flow. I re-ran my control sample and it was fine. no change in analysis.
ccleaned the column and took it off to do other analysis on a different column. reinstalled last week and thats where the sudden RT shift came.
Can this really be due to the column or am i looking at something else?
What is unusual is i run a different method on the same column - same mobile phase but different gradient and flow. And this showed no RT shift at all. Any suggestions at what i'm looking at? I'm worried its the column but i think it may be a method/configuration issue with not seeing any difference on the same column but different method.
thanks in advance!