Retention time calculations for a different column.
Posted: Fri Mar 27, 2009 8:09 pm
I am trying to validate use of 150-x4.6 mm RPHPLC column instead of 125x4 mm column (same stationary phase), with gradient elution. If this was an isocratic separation, the correlation coefficient would be [(150/125)X(4.6/4)^2 = 1.587 calculated based on linear velocity. But I have a gradient.... and it seams to me that the dwell time should be involved as well. Are there calculators available on a web? Is there a way to use a DryLab for that? Thanks...