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Change FPLC to HPLC for ssDNA seperation

http://www.chromforum.org/viewtopic.php?t=10255

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Change FPLC to HPLC for ssDNA seperation

Posted: Mon Mar 23, 2009 2:51 pm
by harish
Hallo,
i have a FPLC AKTA from amersham. I want to use it for speration of single strand DNA 20-100 bases. I have a problem of pressure as it cant go more than 10 Mpa. Is it enough to use a better pump or longer column. I dont see any peaks in the graph. Or there is no option than replacing with new HPLC which is costly.
Please help me in this regard,
Greetings,
Harish.

Re: Change FPLC to HPLC for ssDNA seperation

Posted: Wed May 09, 2012 5:40 am
by megranate16
you should find the suitable column for the ssDNA seperation. and according to the column and packing material requirement choose the equipment.



Lisure Science(suzhou)Co.,Ltd
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Re: Change FPLC to HPLC for ssDNA seperation

Posted: Wed May 09, 2012 10:19 am
by unmgvar
what is the the column and conditions you want to use?
do you want to have a good separation between n to n-1?
are these "end protected" ssDNA?
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