hi, I am looking for a column that can separate chlorinated-amines/amino acid, say Cl-glycine(one hydrogen attached to the N will be replaced by Cl) and other similar chlorinated amino acid/amines.
I am using Tosoh amide 80 now, and I am getting worried as there maybe a Chlorine transfer from Cl-glycine to the stationary phase, that means, I could lose my Cl, which the compound will become maybe glycine again, which could not react with my post column reaction module,(KI solution), so no I3 form, and can't be detected by UV detector. I do see the peak at 4.4min. I am going to try with flow injection to see the on column loss. What do your guys thinking, will this be a big problem, should I switch to other Hilic column or ion exchange, as this group of compounds will not be retained on Reverse phase C18 column. Any suggestions?

I checked the literature, and found out the Glycine has a very close to zero hydrophilic coefficient, (a little bit negative), and lysine has a decent hydrophilic coefficent (2.4), so I guess I will try chlorinated lysine. However, I am still worrying the reaction of the compounds with the cambamoy group on the stationary phase or maybe it wil not happen as the water layer is where the partition happens.

Any ideas?

Really appreciate, my id is "confused amines.".