Wide MS peak at [low] injection...

[ZeroAir]

I am injecting 1uL of a C28 fatty acid standard (2mg/mL in THF) into my HPLC (C18 column, THF -> Hexane gradient, .1mL/min), then passing aliquots of the eluent to a solution of 50:45:5 CHCl3:MeOH:H20 from an isocratic pump using an active splitter. This solution feeds into an ESI-MS. I get this chromatogram:






Does anyone know how I can fix this? Why isn't there a tight gaussian peak? The sub divisions of the peak and baseline are due to the pumping action of the isocratic pump.


Thanks.

Luke

[ZeroAir]

Nevermind - Agilent Rep came by today and we got it sorted. Too low a flow rate.