Chromatography Forum

Hi All,
I have created a 5 level internal calibration and its worked nicely and created good calibration curves for my 6 analytes. The internal standard is included.
However I am unsure what data to put into the mult/divisor/amount std fields in the sample list.
I used level 3 as a test overnight with 4 different info sets:
I used mult of 1 and 100 with internal standard amounts as g's and %.
None seemed to give values close to that in the level3 std.
Any help would be gratefully received.
How do you do intstd quant on Saturn?
Regards
WK

Hi All,
I have since picked up on the tutorial and realised that the internal standard used is at same concentration in samples and standards.
This is a start but there are fields to insert different int std concs in the method for diff calib levels. Also int std field in sample list - somewhere there I must add sample amount too.
As I say calib seemed ok within method - its just numbers at sample list end of things
Any help appreciated.
Regards
WK

Hi All
I've worked the quantitation out but the best results I can get are approx. 2.75-3.00% for a 5.00% standard when run as a sample. %rsd over 5 injections is 2.5% whereas its 0.5% for an equivalent FID run. My analytes are not overloaded on trap either.
Not that good I suppose - I expect its normal for a trap - what is a typical %rsd on a quad?
Regards
WK