Explanation of derivitization sequence
Posted: Fri Dec 20, 2019 9:44 pm
Hello all,
I recently started at a new company and the method I'm working on now (extraction of Histamine Dihydrochloride) has had several predecessors, one of whom developed a derivitization sample prep method in OpenLab.
I've never dealt with derivitization of any kind before, so the mechanics of it are pretty confusing, and I was hoping one of you brainiacs would be able to explain it to me.
As far as I understand, Histamine Dihydrochloride doesn't fluoresce normally, so the o-Phthalaldehyde derivitization needs to take place and to chelate it, which somehow makes it fluoresce. What I really don't understand is how the sample prep was determined. I've attached a screenshot of what I'm talking about.
Any and all help would be greatly appreciated, thanks!
I recently started at a new company and the method I'm working on now (extraction of Histamine Dihydrochloride) has had several predecessors, one of whom developed a derivitization sample prep method in OpenLab.
I've never dealt with derivitization of any kind before, so the mechanics of it are pretty confusing, and I was hoping one of you brainiacs would be able to explain it to me.
As far as I understand, Histamine Dihydrochloride doesn't fluoresce normally, so the o-Phthalaldehyde derivitization needs to take place and to chelate it, which somehow makes it fluoresce. What I really don't understand is how the sample prep was determined. I've attached a screenshot of what I'm talking about.
Any and all help would be greatly appreciated, thanks!