Chromatography Forum

Hi all,
In an agilent chem station, can I subtract one chromatogram from another in order to eliminate specific peaks?
I am using ascorbic acid to prevent oxidation of a plant extract (polyphenols) and the peak from the acid is so large that all else is hardly visible. To further complicate matters, the buffer is using phosphoric acid and at 280nm it causes a substantial drift of the baseline. I see that a baseline can be subtracted but have no idea how and the manual is not helping.
I don't really want to cut off the method before I get to the peak from the ascorbic acid because I also expect peaks following it. Also I will not be able to change the method in mid-project.
Help, please?
Susanne

Plan "A" is to make sure that the ascorbic acid peak isn't hiding any other peaks, then just ignore it. Zoom in tight enough to the baseline so that you can see the peaks of interest. The aa peak will be off scale, but that's OK.

Baseline subtraction is generally only used as a last resort to subtract out undesirable peaks that appear in blank injections and cause some interference with peaks on interest. Subtracting out a large peak would almost certainly lead to more integration problems.

Hi DR,thank you!
pretty much what I figured, but it doesn't hurt to ask, right?

but still, I would like to know how to subtract a baseline, if for no other reason, for future reference, so if someone could point me in the right direction = some book, literature?
Do I need to do this in another software?
Susanne