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Mercaptopurine in Mobile Phase

Posted: Sat Feb 14, 2009 11:27 am
by Nour
would you mind telling me please what is the role of mercaptopurine solution added to mobile phase in the assay of PHENYLMERCURY BORATE.
the mercaptopurine solution is 1mg/ml of mercaptopurine in water. and the mobile phase is a 0.005 M KH2PO4 buffer 500 ml, ACN 495 ml and mercaptopurine solution 5ml. the wave length is 293 nm and the column is a µbondapack C18 300 x 3,9 mm, 10 µm.

one more thing

Posted: Sat Feb 14, 2009 11:28 am
by Nour
can mercaptopurine be replaced by another reagent?