MS Tune Problem with MS5975/6890N

[ak81]

Hi everyone,

I am having a problem when i am performing the tuning on Agilent 5975/6890N. The tune gives me a message saying "Gain at maxium and the high mass is too wide". I called Agilent, and they were saying this could be a problem with my sideboard. I started to see this problem when i first ran the extract from the LDPE strip. I could not get a stable run. The sensitivity could suddenly increase during the analysis. And eveytime, i cleaned my source, the problem will go away. But then, the problem comes back after a few sample run. Could this be a problem with the sample matrix or it really is the electronic problem?

Thanks for your help.

[jh1]

Have you tried using the other filament?


Agilent likes to blame a whole range of MSD issues on the sideboard, many of which turn out to have much simpler solutions (filaments, lack of tuning solution, lose cord or wire, air leak, etc.) so you might consider poking around.

[ak81]

Thanks, jh1.

I have tried different filament, recleaned the source, and retighten all the wires. And I dun think there is an air leak. However, the tune still fails. It has given me a new message "Tune action stopped: -222,â€

[AICMM]

An Agilent FSE that I worked with a couple of weeks ago made mention of tune files becoming corrupted. You might try a different autotune just to see. Instead of standard spectra autotune you could try max sensitivity autotune or vice versa.

Best regards.

[ak81]

Thanks, everyone.

I ended up changing the sideboard. Pricy!!! Now, it works again.